About our contract analysis service

There are three major types of contract analysis services available through KM Assay center.

One is mycotoxin analysis service of food products, one is biological sample analysis, and the other is another analysis.

Please feel free to ask us customized services as we accept samples other than those listed below (including non-food samples) for analysis.

We perform mycotoxin analysis of food products by testing for trichothecene mycotoxins, including DON, NIV, zearalenone, aflatoxin B1 and patulin.

Mycotoxin analysis	method of measurement	Required sample amount	Main types of sample analyzed	Detection limit
Two types of trichothecene mycotoxins	ELISA	Approximately 100g	Grains	DON 100ppb, NIV 50ppb
Zearalenone	ELISA	Approximately 100g	Grains and animal feeds	50ppb
Aflatoxin B1	HPLC	Approximately 100g	Grains and nuts	2ppb
Patulin	HPLC	Approximately 100mL or 100g	Apple juice and the juice of other fruits, fruits	20ppb

For biological sample analysis, we perform analysis that detect the presence of Lipid-related Metabolite Profiling, substance P, TARC, Trp-Man, nitrotyrosin, vitamin C, and NGF in the biological sample.

Biological sample analysis	Method of measurement	Samples analyzed	Required sample amount	Sample pretreatment	Detection limit
Lipid-related metabolite profiling	enzyme method and electrophoresis	Blood serum	Blood serum 2mL or more	Please send us a refrigerated sample of separated blood serum (Do not freeze).	TG,TC　　5mg/dL HDL-C, LDL-C, RemL-C　　1mg/dL
Substance P	ELISA	Blood plasma	Blood plasma 250μL or more	We will send out a stabilizing agent. Please send us a frozen sample of blood plasma that has been separated from drawn blood no more than four hours before sending and which has been mixed with an equal amount of the stabilizing agent.	8.2pg/mL
TARC	ELISA	Blood serum	Blood serum 2mL or more	Please send us a frozen or refrigerated sample of separated blood serum.	10pg/mL
Trp-Man (MPT)	HPLC	Blood serum, urine	Blood serum 1mL or more Urine 1mL or more	Please send us a frozen sample of centrifuged blood serum or collected urine.	Blood serum 0.023μg/mL Urine 0.03μg/mL
Nitrotyrosin	ELISA	Blood plasma	Blood plasma 100μL or more	Please send us a frozen sample of separated blood plasma.	50nM
Vitamin C (Ascorbic acid)	enzyme method	Blood plasma	Please ask us.	Please ask us.	Please ask us.
NGF	ELISA	Blood serum	Blood serum 1mL	Please send us a frozen sample of separated blood serum.	17.7pg/mL

For another analysis, we now perform allergen adsorption testing and lipid peroxides in cooking oils.

another analysis	Method of measurement	Samples analyzed	Required sample amount	Sample pretreatment	Detection limit
Allergen adsorption testing	absorptiometry, electrophoresis	We guide you.	Please ask us	Please ask us	Please ask us
Lipid peroxides	colorimetric method (Hb/MB change)	cooking oils	cooking oils 2g or more	Please send us cooking oils in an airtight container.	1μmol/g

The process flow for our contract analysis service is as follows:

Please contact KM assay Center by e-mail or a fax to request a contract analysis service.

For biological sample analysis, there are items such as stabilizing reagents that we need to send out to our customers before they send in their samples.
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Please fill out the analysis request form and the analysis sample specification form and send these to KM Assay Center along with your samples.
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Although the turnaround time will depend on the sample volume, we can usually mail out the results in approximately two weeks.

If you would like to receive an advance notification of your results, we will send a preliminary report via FAX or e-mail. Please indicate your preference in request form.

After the analysis is complete, we will mail you a bill. Please make a payment once you receive the bill.