Characteristics
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* | Selective solubilization |
| * | Liquid reagent and ready to use with the direct method | |
| * | 15 months after manufacture (stored at 2-8 Degree C) | |
| * | High specificity for LDL Cholesterol | |
| * | Certified traceability by CDC (BQ method) (Centers for Disease Control and Prevention) | |
| * | Excellent correlation with Friedewald formula | |
| * | Specific reactivity for IDL (arteriosclerosis evoking lipoprotein) | |
| * | Non-reactivity for VLDL until 1500mg/dL of Triglyceride |
Measurement Principle
Selective Solubilization method
This measurement uses a unique surfactant to selectively solubilize LDL-C. With cholesterol esterase and cholesterol oxidase, hydrogen peroxide is liberated from LDL-C.Cholesterol esterase releases free cholesterol. Then, cholesterol oxidase releases hydrogen peroxide from free cholesterol, which reacts with 4-AA and HSDA in the presence of peroxidase to generate quinoneimine chromogen. This surfactant solubilizes only LDL, and inhibits other reactions with non-LDL lipoproteins (such as HDL, VLDL and chylomicrons) . The concentration of LDL-C int the sample is determined by absorbance.